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Molecular Biology

FaviconSNBL USA provides a comprehensive range of molecular biology services facilitated by a team with noted expertise in the field. Our team has the experience necessary to assess the biodistribution and/or expression of gene therapy products in studies conducted under Good Laboratory Practices. Services include Droplet Digital PCR (ddPCR), real-time quantitative PCR (qPCR) and real-time reverse transcriptase qPCR (RT-qPCR).

Molecular Biology Endpoints

GLP & MIQE Compliant qPCR & ddPCR

Assay design
Biodistribution/Vector copy number
DCA/Lot release for Nucleic Acid TA
Pathogen screening
Genetic marker screening

RT-qPCR

Transgene expression
Target gene knockout
Off-target modulation of gene expression

Western Blotting for Transgene Expression

Relative expression Densitometry

Pharmacology/Biodistribution

Vector tropism
Gene expression efficiency & kinetics
Vector clearance

Resources

DNA/RNA Isolation and Characterization

QIAGEN Tissuelyser II
Hamilton MicroLab Star Robot
Agilent Bioanalyzer 2100 for DNA & RNA integrity analysis
Direct quantitation by NanoVue spectrophotometer
Fluorescent DNA/RNA quantification
Gel electrophoresis

Tissue Western Blotting

Bio-Rad Chemi-Doc MP high–resolution imager/densitometer

ddPCR/qPCR/RT-PCR

Bio-Rad QX200 AutoDG ddPCR system

Two iQ5 Real-Time PCR systems

Two CFX96 Real-time PCR systems

Contact our Molecular Biology Experts

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